Product info:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Thyroxine(T4) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Thyroxine(T4). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Thyroxine(T4) in the samples is then determined by comparing the OD of the samples to the standard curve.
Research area:
Endocrinology;Hormone metabolism;
Species reactivity:
Cattle
Alternative names:
3,5,3',5'-Tetraiodothyronine; L-Thyroxine; Levothyroxine
Sample type:
serum, plasma and other biological fluids
Detection range:
4.69-300 ng/mL
Sensitivity:
1.42 ng/mL
Standard:
300 ng/mL
Detection method:
Competitive Inhibition
Assay time:
2h
More info:
Email: info@sobekbio.com
Orders:
Email: info@sobekbio.com