Product info:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ComplementFragment 3d(C3d). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to ComplementFragment 3d(C3d). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ComplementFragment 3d(C3d), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ComplementFragment 3d(C3d) in the samples is then determined by comparing the OD of the samples to the standard curve.
Research area:
Immunology
Species reactivity:
Human
Alternative names:
C3d
Sample type:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection range:
7.81-500 ng/mL
Sensitivity:
4.69 ng/mL
Standard:
500 ng/mL
Detection method:
Sandwich
Assay time:
3.5h
More info:
Email: info@sobekbio.com
Orders:
Email: info@sobekbio.com