Product info:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ComplementiC3b(iC3b). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to ComplementiC3b(iC3b). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ComplementiC3b(iC3b), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ComplementiC3b(iC3b) in the samples is then determined by comparing the OD of the samples to the standard curve.
Research area:
Signal transduction;Infection immunity;Immune molecule;
Species reactivity:
Human
Alternative names:
iC3b
Sample type:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection range:
3.13-200 ng/mL
Sensitivity:
1.88 ng/mL
Standard:
200 ng/mL
Detection method:
Sandwich
Assay time:
3.5h
More info:
Email: info@sobekbio.com
Orders:
Email: info@sobekbio.com