Product info:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to E-Cadherin(E-Cad). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to E-Cadherin(E-Cad). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain E-Cadherin(E-Cad), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of E-Cadherin(E-Cad) in the samples is then determined by comparing the OD of the samples to the standard curve.
Research area:
Signal Transduction;Cancer;Developmental Biology
Species reactivity:
Mouse
Alternative names:
CDH1, Arc-1, CD324, CDHE, LCAM, UVO, CAM 120/80, Epithelial Cadherin, Uvomorulin
Sample type:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection range:
62.50-4000 pg/mL
Sensitivity:
37.50 pg/mL
Standard:
4000 pg/mL
Detection method:
Sandwich
Assay time:
3.5h
More info:
Email: info@sobekbio.com
Orders:
Email: info@sobekbio.com