Product info:

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chemokine C-X-C-Motif Ligand 15(CXCL15). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chemokine C-X-C-Motif Ligand 15(CXCL15). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chemokine C-X-C-Motif Ligand 15(CXCL15), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chemokine C-X-C-Motif Ligand 15(CXCL15) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research area:

Immunology

Species reactivity:

Mouse

Alternative names:

C-X-C motif chemokine 15,Chemokine (C-X-C motif) ligand 15,Cxcl15,CXL15,Lungkine,Scyb15,Small inducible cytokine B15,Small inducible cytokine subfamily B member 15,Small-inducible cytokine B15,weche

Sample type:

serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Detection range:

78.13-5000 pg/mL

Sensitivity:

46.88 pg/mL

Standard:

5000 pg/mL

Detection method:

Sandwich

Assay time:

3.5h

More info:

Email: info@sobekbio.com

Orders:

Email: info@sobekbio.com