Product info:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Plasmin-Antiplasmin Complex(PAP). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Plasmin-Antiplasmin Complex(PAP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Plasmin-Antiplasmin Complex(PAP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Plasmin-Antiplasmin Complex(PAP) in the samples is then determined by comparing the OD of the samples to the standard curve.
Research area:
Cardiovascular;Cancer;Cell Biology
Species reactivity:
Mouse
Alternative names:
PAP
Sample type:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection range:
3.13-200 ng/mL
Sensitivity:
1.88 ng/mL
Standard:
200 ng/mL
Detection method:
Sandwich
Assay time:
3.5h
More info:
Email: info@sobekbio.com
Orders:
Email: info@sobekbio.com