Background:

Phosphofructose kinase-2 (PFK-2) belongs to the phosphoglycerate mutase family and is required for the activation of cellular glycolysis. Within the glycolysis pathway, PFK-2 regulates the synthesis and degradation of fructose 2,6-bisphosphate (F2,6BP) by enzymatically catalyzing the phosphorylation of fructose-6-phosphate to form F2,6BP. F2,6BP functions as a potent activator of 6-phosphofructo-1-kinase that can then activate the glycolysis pathway. Various tissue-specific isoforms of PFK-2 are expressed and they are differentially regulated and function as homodimers. A unique isoform, iPFK-2, is induced following proinflammatory stimuli, and it is also constituitively expressed in a variety of carcinoma cell lines, where it leads to an accumulation of intracellular F2,6BP. Activation of PFK-2 cardiac (PFK-2 car) by insulin results from Ser 466 and Ser 483 phosphorylation and requires a PDK-1-activated protein kinase other than PKB.

Alternative names:

PFKFB2; 6-phosphofructo-2-kinase/fructose-2; 6-bisphosphatase 2; 6PF-2-K/Fru-2,6-P2ase 2; PFK/FBPase 2; 6PF-2-K/Fru-2,6-P2ase heart-type isozyme

Clonality:

Polyclonal

Reactivity:

Human,Mouse

Source:

Rabbit

Isotype:

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Immunogen:

Synthesized peptide derived from the N-terminal region of human PFK-2 car. at AA rangle； 1-80

Concentration:

1mg/ml

Applications:

WB, IHC, ELISA

Recommende dilutions:

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Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

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