Background:
Chromatin is the arrangement of DNA and proteins in which chromosomes are formed. Correspondingly, chromatin is formed from nucleosomes, which are comprised of a set of four histone proteins (H2A, H2B, H3, H4) wrapped with DNA. Chromatin is a very dynamic structure in which numerous post-translational modifications work together to activate or repress the availability of DNA to be copied, transcribed, or repaired. These marks decide which DNA will be open and commonly active (euchromatin) or tightly wound to prevent access and activation (heterochromatin). Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine.
Specificity:
This antibody detects endogenous levels of Histone H3 protein only when Methyl at Lys18.
Alternative names:
Histone H3.1; Histone H3A; H3FA; Histone H3B; H3FL; Histone H3C; H3FC; Histone H3D; H3FB; Histone H3E; H3FD; Histone H3F; H3FI; Histone H3G; H3FH; Histone H3H; H3FK; Histone H3I; H3FF; Histone H3J; H3FJ; Histone H3.2; Histone H3/m; Histone H3/o; HIST2H3A; HIST2H3C; H3F2; H3FM; HIST2H3
Species reactivity:
Human,Mouse,Rat
Host:
Rabbit
Ig type:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Immunogen:
Recombinant antibody.
Purification & Purity:
Protein A affinity purified
Molecular weight:
~ 17 kDa
Applications:
WB ICC/IF IHC
Usage:
WB: 1:1000-1:5000 IHC/ICC/IF: 1:50-1:200
Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Precautions:
For research use only, not for use in diagnostic procedure.
More info:
Email: info@sobekbio.com
Orders:
Email: orders@sobekbio.com