Background:
Tight junction protein ZO-1 is a protein that in humans is encoded by the TJP1 gene. This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions. ZO1 plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells.
Specificity:
This antibody detects endogenous levels of ZO1 and does not cross-react with related proteins.
Alternative names:
Tight junction protein ZO-1; Tight junction protein 1; Zona occludens protein 1; Zonula occludens protein 1; TJP1; ZO1
Species reactivity:
Human,Mouse,Rat
Host:
Rabbit
Ig type:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Immunogen:
This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to ZO1.
Purification & Purity:
Peptide affinity purified
Molecular weight:
~ 194 kDa
Applications:
ICC IHC
Usage:
ICC: 1:200
IHC: 1:200
Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Precautions:
For research use only, not for use in diagnostic procedure.
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