The main task of 3D culturing is to prepare cells for further biochemical, molecular or imaging analyses. LifeGel Digestion Kit is a set of enzymes used to release cells cultured on LifeGel. Depending on incubation time a single cells or spheroids are isolated from LifeGel.
Carefully selected combinations of enzymes digest the hydrogel as well as the protein connections between cells, enabling single cells to be recovered. In contrast to classically used trypsin, these enzymes do not digest the membrane proteins necessary for the proper functioning of the cell, so the Digestion Kit is therefore more gentle on cells. Released cells can then be used in further experiments such as gene or protein expression analyses, or they can be re-seeded for further culturing.
LifeGel Digestion Kit contains four 4 mL vials (16 mL in total) containing appropriate enzymes that when added to the LifeGel (in a 1:1 ratio) release cells for further experiments. In general, 16 mL of enzymes are sufficient to digest one culture plate of LifeGel.